COURSE LAYOUTWeek 1 : Introduction to Interactomics and Protein Arrays, High throughput platforms of interactomics: Protein arrays, An introduction to Cell-free protein synthesis, Cell-free synthesis based protein microarrays: PISA and NAPPA, Cell-free synthesis based protein microarrays: MIST, DAPA and Halotag ArraysWeek 2 : Digging deeper into NAPPA: Basic Workflow, Digging deeper into NAPPA: Surface Chemistry, Printing and Assessment, NAPPA Technology and Protein Arrays-I, NAPPA Technology and Protein Arrays-II, Biomarkers: Harnessing the immune system for early detection of disease-IWeek 3 : Biomarkers: Harnessing the immune system for early detection of disease-II, Biomarkers: Harnessing the immune system for early detection of disease-III, NAPPA and its applications in study of antibody immune response in disease and in drug screening-I, NAPPA and its applications in study of antibody immune response in disease and in drug screening-II, NAPPA and its applications in study of antibody immune response in disease and in drug screening-IIIWeek 4 : Using functional proteomics to identify biomarkers and therapeutic targets-I, Using functional proteomics to identify biomarkers and therapeutic targets-II, Conventional label based detection techniques for Protein microarrays, Basics of microarray image scanning Software for Image scanning and data processingWeek 5 : Microarray Data Analysis: Part I, Microarray Data Analysis: Part II, Statistical Analysis-I, Statistical Analysis-II, Novel detection techniques for Protein microarraysWeek 6 : Recombinational cloning and its application for Protein microarrays, Introduction to Bioprinting and Iris™ Optical QC Benefits-I, Introduction to Bioprinting and Iris™ Optical QC Benefits-II,Application of cell free expression protein microarrays in biomarker discovery, Application of protein microarray in biomarker discovery-IWeek 7 : Application of protein microarray in biomarker discovery-II Applications of protein microarrays in Cancer Research-I, Applications of protein microarrays in Cancer Research-II, Application of cell free expression protein microarrays in immunological studies, Applications of protein microarrays in Malaria Research-IWeek 8 : Applications of protein microarrays in Malaria Research-II, Basics and Applications of Reverse Phase Protein Arrays-I, Basics and Applications of Reverse Phase Protein Arrays-II, Basics and Applications of Reverse Phase Protein Arrays-III, Antibody signatures defined by high-content peptide microarray analysisWeek 9 : Demo sessions/ Quiz practice/Application based expts, An overview of label-free technologies-I, An overview of label-free technologies-II, Surface Plasmon Resonance- Principles and Assays-I Surface Plasmon Resonance- Principles and Assays-IIWeek 10: Basics of SPR: Experimental design, Lab session– Basics of SPR: Surface chemistry, Protein immobilization for protein-protein interaction studies, Protein-protein interaction study: Binding analysis Protein-protein interaction study: Kinetics analysisWeek 11: Use of SPR in unravelling domain motif interactions of proteasomal assembly chaperones Protein-small molecule interaction study: Immobilization & binding analysis,Protein-small molecule interaction study: Kinetic analysis,SPR: Interactive Session - I, SPR: Interactive Session - IIWeek 12: An overview of ellipsometry and interferometry techniques, Biomolecular interactions using Bio-Layer Interferometry (BLI)-I, Biomolecular interactions using Bio-Layer Interferometry (BLI)-II Lab session- An introduction to BioLayer Interferometry (BLI) and its applications in protein research, Kinetic analysis of protein-protein interaction using BLI
Prof. Sanjeeva Srivastava