NMR spectroscopy for Chemists and Biologists

COURSE LAYOUT

• Nuclear Spin and Magnetic Moments
• Nuclear Spins in a Magnetic Field Spin
• Lattice Relaxation Spin temperature Resonance
• Absorption of Energyand The NMR Experiment Resonance
• Absorption of Energy and The NMR Experiment Kinetics of Resonance Absorption

• Selection Rules and Line widths
• Bloch equations
• More about relaxation
• More about relaxation Sensitivity

• Instruction to operator Algebra
• Chemical Shift
• Anisotropy ofchemical shifts Learning spectral simulation

• Factors Influencing Isotropic Chemical shifts: Spin Spin Coupling
• Analysis of NMR spectra of molecules
• Learning spectral simulation

• Dynamic Effects in the NMR spectra : Two site exchange
• Collapse of spin multiplets
• Conformational Averaging of J- values
• Analysis of NMR spectra of molecules with J Values

• Principles of Fourier transform
• NMR Theorems on Fourier transforms
• Practical aspects of recording FTNMR spectra
• Free Induction Decay (FID) and the spectrum
• Pulse repetition rate
• Folding of signals
• Acquisition time and the resolution
• Data processing in FT
• NMR Learning of Data processing

• Dynamic range in FTNMR and solvent suppression
• The Nuclear Overhauser Effect - Experimental Schemes,Advanced Treatment
• Steady state NOE and Transient NOE

• Spin Echo Uncoupled spins
• Spin Echo Coupled spins
• Spin-lattice relaxation
• Spin-spin relaxation
• Polarization transfer SPT and INEPT spectralsimulation

• Density matrix, Elements of Density Matrix, Time evolution of density operator
• Time evolution of density operator
• Product operatorformalism

• Segmentation of the time axis
• Two dimensional NMR 2D
• Fourier Transformation in NMR
• Peak shapes in 2D spectrum
• Quadrature detection in two-dimensional NMR

• 2D- resolution/ separation experiments
• Two-dimensional correlation experiments COSY,TDQ-COSY etc TOCSY

• 2D NOESY, 2D ROESY, Heteronuclear COSY,
• The HETCOR pulse sequence HSQC