Experimental Biochemistry

For undergraduate students in the discipline of biochemistry, molecular biology, chemistry and other related biological sciences, the biochemistry laboratory course has become an integral part. It is necessary for students to acquire skills in working with biomolecules in the laboratory. This course is designed to train students with the basic biochemistry laboratory techniques and also introduce some higher level concepts that will prepare them for future research and development projects. The course outline is given for each week. We will introduce each topic and give an overview of the topic and underlying theory. This will be followed by actual demonstration of the experiment. Weekly assignments will be provided and graded.INTENDED AUDIENCE : CHEMISTRY, BIOCHEMISTRY & BIOTECHNOLOGY(Any student with a course in Biochemistry in their curriculum)PRE-REQUISITES : Biochemistry desirablelINDUSTRIES APPLICABLE: Biotech & Pharma companies

Week 1 : Basics of Experimental BiochemistryWeek 2 : Amino Acid Titration and pI determinationWeek 3 : Spectroscopic techniquesWeek 4 : Protein folding and denaturation studiesWeek 5 : Chromatographic techniquesWeek 6 : Gel electrophoresis of DNA and proteinsWeek 7 : Isolation and characterization of proteinsWeek 8 : Enzyme KineticsWeek 9 : Isolation and characterization of DNAWeek 10 : Basics of rDNA technologyWeek 11 : Protein ligand interactionsWeek 12 : Immunoassay techniques