Experimental Biotechnology

In the current MOOCs course I have put effort to briefly discuss different analytical techniques and their potential in solving the scientific problems. We are taking several scientific problems or questions which can be solved by using these techniques. By the end of this course, student will be able to understand: 1. Basics of Good Lab practices. 2. Understanding different analytical techniques and their applications. 3. Specific Scientific questions and their solutions. 4. Designing new experiments. INTENDED AUDIENCE :PG/PhDPREREQUISITES : General Biology 10+2INDUSTRIES SUPPORT :Biocon https://www.biocon.com/ Jubilant Life Sciences www.jubl.com/ Shantha Biotechnics Ltd Panacea Biotec Other companies related to biotechnology

Week 1: Basics of Laboratory Research:Good Lab Practices, Concept of buffering and Preparation of Solutions and ReagentsWeek 2: Electrophoresis (Part 1):Basic Concept of Electrophoresis, performance of electrophoresis and its applicationsWeek 3:Electrophoresis:Horizontal Gel electrophoresis, discussion about scientific questions and related experiments to solve them utilizing electrophoresisWeek 4: Chromatography (Part I): Analytical techniques in purification of biomolecules; Column chromatography, HPLC.Week 5: Chromatography (Part II) : Analytical techniques in purification of biomolecules; Column chromatography, HPLC.Week 6: Chromatography (Part III) : Discussion about scientific questions and related experiments to solve them utilizing chromatographyWeek 7:Immunology Techniques: Immunological tools, Antibody Generation and Purification, ELISA, Radial Immuno-diffusion, Western blotting and Immuno-precipitation. Week 8: Cell Biology Techniques (Part 1): Basics of cell-culture, isolation of cells from tissue and fractionation.Week 9: Cell Biology Techniques (Part 2): Immuno-localization, cell sorting, and discussion about scientific questions and related experiments to solve them utilizing cell biology techniques. Week 10:Molecular Biology (Part 1):Primer designing, Polymerase chain reaction, Different variants of PCR and their applications.Week 11:Molecular Biology (Part 2) Blotting Techniques, DNA and protein sequencing techniques and their applicationsWeek 12:Summary and Conclusions